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1. Genetic code
a. What amino acid(s) do the following codons code for?
i. 3’ CAU 5’
ii. 5’ CAA 3’
b. What codon(s) encode for the following amino acids? Include directionality.
i. Asp
ii. Cys
c. A tRNA has the anticodon 5’ IGA 3’. (1) What codon(s) can it bind to? Include directionality. (2) What amino acid(s) will be attached to this tRNA?
(1) (2)
d. The sequence of a given mRNA is 5’ AUGUAG 3’. If the amino acid sequence of the protein made from this mRNA is Met-Cys-Val, this would mean the genetic code is (OVERLAPPING/NON-OVERLAPPING).
2. Stop!
a. Which of the following best describes why splicing is important for the detection of a nonsense mutation?
A. An mRNA that is not spliced cannot be exported from the nucleus.
B. An mRNA that is not spliced will not have exon junction complex proteins associated with it.
C. An mRNA that is not spliced cannot be bound by the ribosome.
b. The Salmon gene is GFP tagged in a human tissue culture cell line. One of the cells in the line acquires a nonsense mutation in the Salmon gene.