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CHEM3121 Chemical Biology
Generic skills plus
PROJECT L: Computing in Chemical Biology
EXPERIMENT L1: ANALYSING REACTION KINETICS – AN INTRODUCTION TO COMPUTER DATA
ANALYSIS
EXPERIMENT L2: STEADY-STATE ENZYME KINETICS
EXPERIMENT L3: BIOINFORMATICS
Project L
Computing in Chemical Biology
CONTENTS
Page
L1 Analysing Reaction Kinetics – An Introduction to Computer Data Analysis 2
L2 Steady-State Enzyme Kinetics 7
L3 Further Investigations of Computing in Chemical Biology
- Bioinformatics 16
- Your Own Investigations 19
AdobeStock – USYD licence
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EXPERIMENT L1: ANALYSING REACTION KINETICS - AN
INTRODUCTION TO COMPUTER DATA ANALYSIS
Aim
The aim of this experiment is use computer methods to investigate reaction kinetics.
Introduction and Background
This experiment is designed to familiarise you with the program Excel. It is also aimed to introduce
you to the subject of chemical kinetics, which allows one to study how chemical reactions occur. To
complete this computer-based experiment, you will need access to Excel and a copy of the files
containing the sample data. Submission of this experiment will be in the form of an electronic
notebook submissions which can be accessed via the Canvas page for this project.
Experimental chemical kinetics usually proceeds by analysing reactant or product concentrations as a
reaction proceeds by the integrated rate law method. The integrated rate law expresses the
concentration of one reactant or product as a function of time. It may be derived from the differential
rate law (rate as a function of concentration) by integration. Two examples are shown below:
First order reaction: Second order reaction:
A → B 2A → B
Differential Rate Law:
−
[]
= [] −1
2
[]
= []2
⇒ −∫
[][][][]0 = ∫ 0 ⇒ −∫ [][]2[][]0 = 2 ∫ 0
⇒ −|[]| [] []0 = ||0 ⇒ − �− 1[]�[]0[] = 2||0
Integrated Rate Law:
⇒ [] = []0 − ⇒ 1[] = 1[]0 + 2
Before you start the experiment answer the following question. How should the experimental kinetic
data be plotted to obtain a straight line and obtain the value of the rate constant for:
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a) a first order reaction?
b) a second order reaction?